The objective of this study was to investigate the profiles of Mycoplasma hyopneumoniae (MH) infection at different ages of pig in a sow – finishing herd using serological and molecular methods. A total of 30 study piglets were born from non-vaccinated sows with MH. They were injected one-dose of inactivated MH vaccine at the 10th week. | 22 Nong Lam University Ho Chi Minh City Infection status of Mycoplasma hyopneumoniae in experimental pigs at a commercial farm Huyen T. N. Bui Hien T. Le amp Toan T. Nguyen Faculty of Animal Science and Veterinary Medicine Nong Lam University Ho Chi Minh City Vietnam ARTICLE INFO ABSTRACT Research Paper The objective of this study was to investigate the profiles of Mycoplasma hyopneumoniae MH infection at different ages of pig Received March 05 2020 in a sow finishing herd using serological and molecular methods. Revised May 11 2020 A total of 30 study piglets were born from non-vaccinated sows with MH. They were injected one-dose of inactivated MH vaccine Accepted June 09 2020 at the 10th week. MH infection status was evaluated by using ELISA to detect MH antibodies from blood samples and PCR to detect MH DNA in nasal swabs or oral fluid samples every other Keywords weeks from newborn to slaughter time. The results of this study showed that PCR positive proportions were low at 1st -2nd week Antibodies 7-13 then increased significantly during 5th -7th week 73-79 ELISA and reduced at 8th week 33 finally became negative after 13th Infection ratio week of age. This pattern corresponds to the one of antibody level. Mycoplasma hyopneumoniae MH In particular the level of maternal antibodies against MH was PCR very high due to maternal immunity then decreased gradually to negative at 7-8 weeks of age and finally increased gradually from 13 weeks of age to all positive at 25 weeks of age. In conclusion the result showed that in this herd MH might invade pigs by the time of 5-7 weeks of age after maternal immunity disappears and Corresponding author humoral response can overcome the infection at week 13. This should be noted to have appropriate strategies to control MH at Bui Thi Ngoc Huyen the farm. Email Cited as Bui H. T. N. Le H. T. amp Nguyen T. T. 2020 . Infection status of Mycoplasma hyop- neumoniae in experimental pigs at a commercial farm. .
