Nghiên cứu này được tiến hành với mục đích thủy phân sụn khớp chân gà với xúc tác enzyme Protamex nhằm thu axit amin. Thành phần hóa học của sụn khớp chân gà được phân tích, kết quả phân tích cho thấy hai thành phần chính trong chất khô của sụn khớp chân gà là lipid (19,72 ± 0,05%) và protein (13,34 ± 0,08%). | Trường Đại học Nông Lâm TP. Hồ Chí Minh 73 Screening of factors influencing the hydrolysis reaction of chicken leg cartilage with Protamex enzyme as a catalyst Cuong V. Bui1 Kim Chi T. Nguyen1 amp Dong X. Bui2 1 Department of Food Technology Faculty of Chemical Engineering University of Science and Technology The University of Da Nang Da Nang Vietnam 2 Department of Bio-Technology Faculty of Chemical Engineering University of Science and Technology The University of Da Nang Da Nang Vietnam ARTICLE INFO ABSTRACT Research Paper The objective of this research was to use Protamex enzyme as a cat- alyst to hydrolyze chicken leg cartilage for production of amino acid Received May 29 2020 hydrolysate. The proximate composition of chicken cartilage was analyzed. Revised July 10 2020 The results indicated that the major components in the dry matter of Accepted August 21 2020 chicken leg cartilage were lipid and protein . The main parameters affecting the hydrolysis reaction of chicken leg cartilage with Protamex enzyme were selected for investigation Keywords reaction temperature o C pH enzyme ratio based on the weight of substrate reaction time min and the ratio of reaction volume v v . Amino acid yield The significant difference was analyzed by ANOVA One-Way to identify the optimal point of each parameter toward amino acid yield. The results Chicken cartilage revealed that the hydrolysis degree and amino acid yield reached the Hydrolysis reaction maximal values of and respectively when Hydrolysis yield the reaction temperature pH enzyme ratio reaction time and ratio of Protamex enzyme reaction volume v v were 40o C 4 40 min and 1 18 respectively. Corresponding author Bui Viet Cuong Email bvcuong@ Cited as Bui C. V. Nguyen K. C. T. amp Bui D. X. 2020 . Screening of factors influencing the hydrolysis reaction of chicken leg cartilage with Protamex enzyme as a catalyst. The Journal of Agriculture and Development 19
