Detection and screening of genes related to biosynthesis of substances with protease inhibitory activity from microorganisms associated with sponge collected from the Mien trung sea of Vietnam by metagenomics method. Then, selective gene, expression of in vitro and determination of activity of the expressed protein. | MINISTRY OF EDUCATION VIETNAM ACADEMY AND TRAINING OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLOGY ----------------------------- Tran Thi Hong SCREENING AND EXPRESSION OF A GENE ENCODING PROTEASE INHIBITOR PROTEIN OF MICROORGANISMS ASSOCIATED WITH THE SPONGE Spheciospongia vesparium COLLECTED FROM MIENTRUNG SEA VIETNAM Major Biotechnology Code 9420201 SUMMARY OF BIOTECHNOLOGICAL DOCTORAL THESIS Ha Noi - 2020 The work was completed in Graduate university of sience and techology- Viet Nam academy of science and technology. Science instructor 1 . Pham Viet Cuong Science instructor 2 . Nguyen Thi Kim Cuc Reviewer 1 Reviewer 2 Reviewer 3 The thesis will be defended in front of the Academy Thesis Evaluation Council meeting at Graduate university of sience and techology- Viet Nam academy of science and technology at . hour . date . month . year 20. The thesis can be found at - Library of Academy of Science and Technology -Vietnam National Library 1 PREAMBLE The urgency of the subject Protease inhibitors PIs an agent that can cause protease to lose its ability to hydrolyze proteins into peptides are applied in many fields especially in medicine such as antiretroviral therapy for fibrinolysis angiotensin inhibitors in the treatment of hypertension prevent the replication of some viruses HIV hepatitis C . . Examples of some protease inhibitors are saquinavir Brand name Invirase and ritonavir Brand name Novir used mainly in the treatment HIV AIDS. They are used as part of a multi-drug cocktail and have been shown to significantly reduce the level of HIV virus in the blood. Therefore the screening and detection of new protein molecules with specific inhibitory activity of different target proteases related to diseases is an interesting research direction. Sponge and its associated microorganisms are considered a source to detect and exploit new biologically active compounds for drug manufacture peptides with protease .
