The present study investigated how mouse fibroblasts changed under microgravity (SMG) conditions ( | ACADEMIA JOURNAL OF BIOLOGY 2020 42 3 129 134 DOI 2615-9023 EVALUATING THE EFFECTS OF SIMULATED MICROGRAVITY ON MOUSE FIBROBLAST Hoang Nghia Son1 2 Hoang Nguyen Quang Huy1 Tran Thi Bich Tram3 Ly Ngoc Cang1 Ho Nguyen Quynh Chi1 2 Truong Xuan Dai1 2 Le Ngoc Phuong Thanh1 Truong Thi Han1 Nguyen Thai Minh Han1 Le Thanh Long1 2 1 Animal Biotechnology Department Institute of Tropical Biology VAST Vietnam 2 Biotechnology Department Graduate University of Science and Technology VAST Vietnam 3 University of Science Vietnam National University in Ho Chi Minh City Received 24 March 2020 accepted 20 September 2020 ABSTRACT The present study investigated how mouse fibroblasts changed under microgravity SMG conditions lt 10-3 G simulated by 3D clinostat. Results showed that SMG condition markedly reduced the proliferation of mouse fibroblasts significantly reducing the nuclear area and intensity. Compared to the control group the mouse fibroblasts ratio of the SMG group was higher in the G0 G1 phase but lower in the S phase and G2 M phase. The ratios of early and late apoptotic cells were also higher in the SMG group. The mouse fibroblasts under SMG conditions exhibited a reduction of β-Actin and α-Tubulin 3 expressions compared to the control group. These results suggested that the SMG condition diminished the proliferation and downregulated cytoskeletal protein expression of mouse fibroblasts. Keywords Cell cycle mouse fibroblast proliferation simulated microgravity. Citation Hoang Nghia Son Hoang Nguyen Quang Huy Tran Thi Bich Tram Ly Ngoc Cang Ho Nguyen Quynh Chi Truong Xuan Dai Le Ngoc Phuong Thanh Truong Thi Han Nguyen Thai Minh Han Le Thanh Long 2020. Evaluating the effects of simulated microgravity on mouse fibroblast. Academia Journal of Biology 42 3 129 134. https 2615-9023 Corresponding author email lelongvast@ 2020 Vietnam Academy of Science and Technology VAST 129 Hoang Nghia Son et al. INTRODUCTION .
