In the present investigation, we have described optimization of environmental and nutritional requirements for maximum biosynthesis of ADI from bacterium Pseudomonas aeruginosa PS2. We observed that batch time 25 h, inoculum age 20 h, 8% (v/v) inoculum size, pH and temperature 37 °C were found as the most suitable operating conditions for ADI production. Galactose, peptone, KH2PO4 and L-arginine were found as the best carbon, nitrogen, mineral ion and inducer for ADI production, respectively. |
