In the past decades, next generation sequencing has improved our understanding of bacterial diversity in soil (Simon and Daniel, 2011). Nguyen et al. (2016) used 454 pyrosequencing to detect bacterial community in Korean ginseng cultivated soil in Korea. But the high cost of 454 pyrosequencing tools have limited small laboratories‟ access. Due to that, in 2001 Illumina developed MiSeq which have enabled deep sequencing of microbial communities at a lower cost (Caporaso et al., 2012). Thus, the aim of the present study was to Miseq to investigate bacterial community and diversity in VNG cultivated soil in the Ngoc Linh Mountain. We can detect bacterial population in soil at relative speed, and the ability to detect uncultivable organisms. We further predicted functional profiles from obtained 16S rRNA data. |
