Bài viết trình bày kỹ thuật realtime PCR đa mồi có độ nhạy, độ đặc hiệu cao, thời gian cho kết quả ngắn, đồng thời phát hiện cùng lúc 19 loại virus và 7 loại vi khuẩn gây bệnh. Nghiên cứu này xác định tỷ lệ nhiễm một số vi khuẩn, virus ở trẻ viêm phổi tái diễn có suy hô hấp bằng kĩ thuật multiplex real-time PCR tại Bệnh viện Nhi Trung ương. | Journal of Pediatric Research and Practice Vol. 5 No. 1 2021 25-30 Research Paper Ratio of Bacteria and Virus Infections in Children Having Recurrent Pneumonia with Respiatory Failure Hospitalized at The Vienam National Children s Hospital Pham Thi Thanh Tam Le Thanh Hai Phung Thi Bich Thuy Lê Ngoc Duy Nguyen Thi Nga Do Quang Vy Dinh Thi Hong Dinh Thi Thu Phuong Vietnam National Children s Hospital No 18 879 La Thanh Dong Da Hanoi Vietnam Received 31 August 2020 Accepted 14 February 2021 Abstract Background Purpose Multi-primer realtime PCR technique has high sensitivity high specificity short result time simultaneously detecting 19 viruses and 7 types of bacteria at the same time. This study is to determine the prevalence of some bacteria and viruses in children with recurrent pneumonia has respiratory failure by multiplex real-time PCR at the Vietnam National Children s Hospital. Methods 95 patients 2 months - 60 months with recurrent pneumonia with respiratory failure from May 2019 to April 2020. A prospective study was conducted on convenient sample size. Results The rate of bacteria was 68 4 of which H. influenzae infection accounted for 35 8 accounted for 22 1 atypical bacteria accounted for 9 5 mainly Mycoplasma Pneumonia. Whooping cough only accounts for . The rate of virus was 42 1 mainly Adenovirus Rhinovirus and RSV . The rate of co- infection is 23 2 most of them are co-infection with bacteria - virus 10 5 co- infection with bacteria - bacteria accounts for 9 5 . Virus-virus co-infection only accounts for 3 2 . Conclusion Most children with severe recurrent pneumonia have Gram-negative infections. Nearly half of them co-infected with viruses or other bacteria. The common agents that cause recurrent pneumonia are H. influenzae S. pneumoniae and Adenovirus. Keywords respiratory failue recurrent pneumonia. ____ Corresponding author. E-mail address ochot1985@ https 25 . Tam et .
