Analysis and comparison of the transcriptome of human hepatocytes infected with the Yellow Fever virus to verify the transcriptional profile of both genotypes, in response to viral infection. The sample used corresponds to strains of YFV code H622205, from the state of Goiás-GO (Brazil), a fatal human case isolated from the patient's blood and serum. The techniques of cell cultures, extraction of viral RNA, sequencing and analysis of transcriptome, as well as the pre-processing of both the RNA and transcriptome libraries, mapping and abundance of transcripts and statistical analyzes were all performed in the Arbovirology Section of the Institute Evandro Chagas. |
