]The study was conducted to explore the cryoprotective effect of L-proline on sheep oocyte vitrification. In vitro matured sheep oocytes (n=836) were vitrified by open pulled straw method in vitrification solution consisting of 15 per cent (v/v) DMSO+ 15 per cent (v/v) EG + sucrose () + 20 per cent FBS in TCMh (Group I) and per cent (v/v) DMSO + 10 per cent (v/v) EG + sucrose () + 20 per cent FBS in TCMh supplemented with 2M L-proline (Group II). |