Microbial transglutaminase (MTGase) is an enzyme widely used in food industry. In this study, MTGases had been applied to produce fresh cheese made from whole milk powder. A set of 18 experiments was carried out to evaluate the influence of factors in reconstituted milk treatment with MTGase. A response surface methodology was applied to evaluate quality properties of fresh cheese via objective functions of hardness, yield, protein content, total solid content and sensory evaluation score. In this model, three factors were enzyme concentration ( - U/g protein), temperature (30 - 60oC), reaction time ( - 6 h). |
