The present research was focused in standardizing the callus induction technique and studies the behaviour of callus in secondary metabolite production in Andrographis echioides (L.) Nees. In this study, the leaf bits of Andrographis echioides responded positively for callus induction. Earliest ( days) and profuse callusing was observed on MS medium supplemented with 2, 4-D ( mgl-1 ) + NAA ( mgl-1 ) + BAP ( mgl-1 ). |