Keeping the background in view, the present study was designed to determine the antibacterial action of enrofloxacin alone and in combination with phytochemicals capsaicin, theobromine, glycyrrhetenic acid and glycyrrhizic acid against S. aureus ATCC 25923, ATCC 25922, K. pneumoniae ATCC 700603 and P. aeruginosa ATCC 27853. | 2020 9 11 1053-1058 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 11 2020 Journal homepage http Original Research Article https In vitro Studies on Potentiation of Enrofloxacin by Phytochemicals Srividya Gullapudi1 G. S. Rao1 P. Ravikumar1 Muralidhar Metta2 and V. Ramadevi3 1 Department of Veterinary pharmacology amp Toxicology CVSC Proddatur YSR Kadapa district Andhrapradesh-516360 India 2 Department of Animal Genetics and Breeding 3Dept of Veterinary Pathology SriVenkateswara Veterinary University Tirupati India Corresponding author ABSTRACT Enrofloxacin an antimicrobial fluoroquinolone is most commonly used against majority of gram negative bacterial and mycoplasma infections in majority of livestock. Indiscriminate Keywords usage of enrofloxacin in clinical practice leads to resistance development to this quinolne Enroloxacin drug. Among the various pathways of resistance efflux pump mediated drug resistance is Capsaicin one of the important pathways identified in the recent past. Phytochemicals namely Theobromine theobromine glycyrrhetenic acid and glycyrrhizic acid and capsaicin were identified as Efflux pump MIC efflux pump inhibitors. Phytochemicals which possess efflux pump inhibitory activity if combined with classical antimicrobial agents reduces the development of resistance and Article Info also improves their therapeutic efficacy. Interaction between enrofloxacin and capsaicin theobromine glycyrrhetenic acid and glycyrrhizic acid were studied by determining MIC Accepted and MBC against and following CLSI 10 October 2020 guidelines. There is a significant decrease in MIC and MBC values of enrofloxacin in the Available Online presence of phytochemicals. In conclusion Synergisitic interaction of efflux protein 10 November 2020 inhibitory phytochemicals capsaicin theobromine .
