Tối ưu hoá điều kiện trích ly flavonoids từ lá diếp cá

Mục tiêu của nghiên cứu này nhằm sử dụng enzyme cellulase trong quá trình trích ly flavonoids từ lá diếp cá (Houttuynia cordata Thumb) và tối ưu hóa các yếu tố ảnh hưởng đến hiệu suất trích ly. Mời các bạn cùng tham khảo! | Trường Đại học Nông Lâm TP. Hồ Chí Minh 51 Optimization of extraction conditions of flavonoids from Houttuynia cordata Thumb leaves Phung M. Phan Phuc T. D. Nguyen Phuc H. Nguyen Vo T. T. Vo amp Anh T. Vu Faculty of Chemical Engineering and Food Technology Nong Lam University Ho Chi Minh City Vietnam ARTICLE INFO ABSTRACT Research Paper This study was conducted to investigate the application of cellulase enzyme in the extraction of flavonoids from Houttuynia cordata Received February 26 2021 Thumb leaves and optimize the extraction conditions. Independent Revised April 01 2021 variables including enzyme concentration 25 - 100 µg mL temper- Accepted April 08 2021 ature 30 - 50o C time 30 - 120 min and ratio of raw materials to enzyme 1 15 - 1 30 g mL were investigated. Extraction conditions Keywords of flavonoids were designed according to Central Composite Design - Uniform Precision CCD a response surface methodology using a Cellulase software JMP Pro version 13. The results indicated that the optimal extraction conditions were found to be enzyme concentration Flavonoids Houttuynia cordata Thumb µg mL temperature 41o C time 90 min and the ratio of material to the enzyme 1 26 g mL . Under such conditions the Response surface methodology highest content of flavonoids mg g dry matter was obtained and validated. It can be concluded that the flavonoids can Corresponding author be optimally extracted under the optimal extraction conditions with assistance of cellulase. Vu Thuy Anh Email vuthuyanh@ Cited as Phan P. M. Nguyen P. T. D. Nguyen P. H. Vo V. T. T. amp Vu A. T. 2021 . Optimization of extraction conditions of flavonoids from Houttuynia cordata Thumb leaves. The Journal of Agriculture and Development 20 2 51-61. Tạp chí Nông nghiệp và Phát triển 20 2 52 Trường Đại học Nông Lâm TP. Hồ Chí Minh Tối ưu hoá điều kiện trích ly flavonoids từ lá diếp cá Phan Minh Phụng Nguyễn Thị Diễm Phúc Nguyễn Hữu Phúc Võ Thị Tường .