Pathogenesis-related proteins (PR-proteins) are induced in response to environmental stresses such as osmotic and drought stress, wounding, microbial infections and treatment with specific plant hormones and elicitors. These proteins are classified into several groups (PR-1 through PR-17) based on their amino acid sequence and biochemical functions. The present study focuses on prediction, isolation, over-expression and analysis of the antifungal activities of the thaumatin-like proteins (. PR-5) in the model legume M. truncatula var. truncatula. Analysis of M. truncatula genome sequence, available freely on the NCBI website, indicated the presence of at least 15 PR-5 Open Reading Frames (ORFs), 5 of them (dubbed TLP-1, -2, -3, -4 and -5) were selected for this study. |
