The osteogenic differentiation markers BMP2, BMP4, COL1A1, OPN, OCN and PF4 evaluated using RT-PCR. The tumor dimensions in the hMSCs group were significantly higher than those of the remaining groups (p | Turkish Journal of Biology Turk J Biol 2021 45 301-313 http biology TÜBİTAK Research Article doi biy-2101-50 The promising effects of BMP2 transfected mesenchymal stem cells on human osteosarcoma 1 2 3 4 3 5 6 7 Ahmet Sinan SARI Emre DEMİRÇAY Ahmet ÖZTÜRK Ayşen TERZİ Erdal KARAÖZ 1 Department of Orthopedics and Traumatology Faculty of Medicine Başkent University Ankara Training and Research Hospital Ankara Turkey 2 Department of Orthopedics and Traumatology Faculty of Medicine Başkent University Istanbul Training and Research Hospital İstanbul Turkey 3 Stem Cell and Gene Therapy Research and Application Center Kocaeli Turkey 4 Department of Pathology Faculty of Medicine Başkent University Ankara Training and Research Hospital Ankara Turkey 5 Istinye University School of Medicine Department of Histology and Embryology İstanbul Turkey 6 Istinye University Center for Stem Cell and Tissue Engineering Research and Practice İstanbul Turkey 7 Liv Hospital Center for Regenerative Medicine and Stem Cell Manufacturing LivMedCell İstanbul Turkey Received Accepted Published Online Final Version Abstract Selective targeting of transfected mesenchymal stem cells MSCs carrying specific antioncogenes to the tumor was suggested as a treatment option. Bone morphogenetic protein-2 BMP2 was shown to inhibit the proliferation and aggressiveness of osteosarcoma OS cells. Here we aimed to assess the homing efficiency of intraperitoneally administered hMSCs transfected with BMP2 to the tumoral site and their effects on OS using an orthotopic xenograft murine model. Orthotopic xenograft murine model of OS in six- week-old female NOD SCID mice using 143B cells was established. hMSCs transfected with BMP2 BMP2 hMSC were used. In vivo experiments performed on four groups of mice that received no treatment or intraperitoneally administered BMP2 hMSCs and BMP2 hMSCs. Histopathological and immunohistochemical studies were used to
