A fast and easy method was validated for simultaneous determination of ciprofloxacin hydrochloride monohydrate (CP), levofloxacin hemihydrate (LV), and N-acetylcysteine (NAC) in samples. The analysis was performed on a C18 column (250 × , 5µm) (Inertsil ODS-3V) using an isocratic elution method with a mobile phase composed of 25mM KH2PO4 (pH ) and methanol (72:28, v/v) at a flow rate of 1mL/min. UV detection was performed at 214nm for NAC and 293nm for CP and LV. The method was validated for linearity, accuracy, precision (repeatability and reproducibility), specificity, sensitivity, and stability. |
