In this study, silver nanoparticle (AgNP) synthesis was carried out using Onosma sericeum Willd. aqueous extract for the first time, with a simple, economical, and green method without the need for any other organic solvent or external reducing or stabilizing agent. A variety of AgNPs, all of different particle sizes, were synthesized by controlling the silver ion concentration, extract volume, temperature, and pH. It was determined that the optimum conditions for AgNP synthesis were 1 mM AgNO3 , pH 8, 25 °C, 20 g/200 mL extract, silver nitrate, and extract ratio 5:1 (v/v). The AgNPs were defined using UV-Vis spectroscopy, field emission scanning electron microscopy (FESEM), energy dispersive X-ray analysis (EDAX), transmission electron microscopy (TEM), and X-ray diffraction (XRD). | Turkish Journal of Chemistry Turk J Chem 2020 44 1587-1600 http chem TÜBİTAK Research Article doi kim-2007-1 Biosynthesis of silver nanoparticles using Onosma sericeum Willd. and evaluation of their catalytic properties and antibacterial and cytotoxic activity 1 2 Selda DOĞAN ÇALHAN Mustafa GÜNDOĞAN 1 Department of Pharmaceutical Biotechnology Faculty of Pharmacy Mersin University Mersin Turkey 2 Department of Pharmaceutical Technology Faculty of Pharmacy Mersin University Mersin Turkey Received Accepted Published Online Final Version Abstract In this study silver nanoparticle AgNP synthesis was carried out using Onosma sericeum Willd. aqueous extract for the first time with a simple economical and green method without the need for any other organic solvent or external reducing or stabilizing agent. A variety of AgNPs all of different particle sizes were synthesized by controlling the silver ion concentration extract volume temperature and pH. It was determined that the optimum conditions for AgNP synthesis were 1 mM AgNO3 pH 8 25 C 20 g 200 mL extract silver nitrate and extract ratio 5 1 v v . The AgNPs were defined using UV-Vis spectroscopy field emission scanning electron microscopy FESEM energy dispersive X-ray analysis EDAX transmission electron microscopy TEM and X-ray diffraction XRD . The particle size distribution and zeta potential measurements of the AgNPs were measured using the dynamic light scattering DLS technique. It was determined that the AgNPs with a particle size of less than 10 nm showed a higher catalytic effect in the reduction of 2-nitrobenzenamine. It was also found that these nanoparticles had a cytotoxic effect on the MCF-7 breast cancer cell line depending on dosage and time. The resulting IC50 values were between µg mL and µg mL. Furthermore the biosynthesized AgNPs showed effective antibacterial activity against the Acinetobacter baumannii bacteria. The .
