Within the scope of the European Union Water Framework Directive, diatom assemblages as biological quality components were used to evaluate the ecological status of 12 surface waters in the Araban-Yavuzeli catchment between October 2013 and October 2014. A total of 75 diatom species were identified during the study period. Amphora ovalis, Denticula elegans, Gomphonema parvulum, Gomphonema truncatum, and Navicula cryptocephala were commonly observed. The relationship between diatom assemblages and environmental factors was assessed by canonical correspondence analysis and weighted average regression. Dissolved oxygen, temperature, nitrate, biological oxygen demand, lead, and copper played significant roles in the distribution and composition of diatom assemblages (P | Turkish Journal of Botany Turk J Bot 2019 43 597-607 http botany TÜBİTAK Research Article doi bot-1901-32 Bioassessing ecological status of surface waters in the Araban-Yavuzeli catchment Turkey application of diatom indices Abuzer ÇELEKLİ Fatma BİLGİ Department of Biology Faculty of Art and Science Gaziantep University Gaziantep Turkey Received Accepted Published Online Final Version Abstract Within the scope of the European Union Water Framework Directive diatom assemblages as biological quality components were used to evaluate the ecological status of 12 surface waters in the Araban-Yavuzeli catchment between October 2013 and October 2014. A total of 75 diatom species were identified during the study period. Amphora ovalis Denticula elegans Gomphonema parvulum Gomphonema truncatum and Navicula cryptocephala were commonly observed. The relationship between diatom assemblages and environmental factors was assessed by canonical correspondence analysis and weighted average regression. Dissolved oxygen tem- perature nitrate biological oxygen demand lead and copper played significant roles in the distribution and composition of diatom assemblages P lt . The ecological status of the stations in Araban and Yavuzeli was characterized using the trophic index-Turkey TIT trophic index TI and eutrophication and or pollution index-diatom EPI-D indices. According to the TIT Ardıl Creek1 had very good ecological conditions and was characterized by the occurrence of pollution-sensitive diatom taxa . Cymbella affinis En- cyonema silesiacum Navicula radiosa indicated by multivariate analyses. The significant positive correlation of the TIT P lt r with logPO4 indicated that TIT may be used as an appropriate diatom metric to assess the ecological status of surface waters in the Araban-Yavuzeli catchment. Key words Bioassessment diatoms Araban-Yavuzeli catchment trophic index-Turkey 1. Introduction .