Cynodon dactylon × Cynodon transvaalensis is one of the most important turfgrasses. Sequencing the C. dactylon × C. transvaalensis mitochondrial genome can help us learn more about its genomic composition and allow further study of the population genetics, taxonomy, and evolutionary biology of Poaceae plants and other related species. Here the C. dactylon × C. transvaalensis mitochondrial genome was sequenced using Illumina HiSeq combined with PacBio sequencing technology, and the map of the mitochondrial genome was constructed after de novo assembly and annotation. The C. dactylon × C. transvaalensis mitochondrial genome has 366,612 bp and contains 53 genes, including 31 protein-coding genes, 3 rRNA genes, and 16 tRNA genes. |