The purified L-asparaginase is monomeric with a molecular mass of 57 kDa and exhibited optimum activity at pH and 37 °C, which is relatively close to the human body's internal environment. The purified Lasparaginase showed high affinity and catalytic efficiency towards its natural substrate L-asparagine with Km and Vmax of mM and U/mL, respectively, and did not exhibit any intrinsic glutaminase activity |