The present research was carried out to determine the anatomical features of leaves of Alangium salviifolium and their endophytic fungal association. Alangium salviifolium is an important medicinal plant with various activities reported. It is suggested that the medicinal property of the plant is either because of their endophytes or the endophytes inherit the medicinal properties from their host plant. Hence the detailed study of plant- microbe interaction is important. | ANATOMICAL STUDIES ON THE ASSOCIATION OF ENDOPHYTIC FUNGI AND THEIR ISOLATION FROM ALANGIUM SALVIIFOLIUM . Wangerin S. Bavya 1 S. Sahaya Sathish1 S. Dominic Rajkumar2 P. Vijayakanth3 V. Thangarajan1 and R. Kavitha1 Address es 1 Department of Botany St. Joseph s College Tiruchirappalli - 620 002 Affiliated to Bharathidasan University Tamilnadu India. 2 Department of Botany St. Andrew s College Gorakhpur - 273 001 Uttar Pradesh India. 3 Department of Botany Aringar Anna College of Arts and science Krishnagiri-635 001 Tamilnadu India. Corresponding author bavyagayathri@ https ARTICLE INFO ABSTRACT Received 23. 7. 2020 The present research was carried out to determine the anatomical features of leaves of Alangium salviifolium and their endophytic fungal Revised 11. 5. 2021 association. Alangium salviifolium is an important medicinal plant with various activities reported. It is suggested that the medicinal Accepted 18. 5. 2021 property of the plant is either because of their endophytes or the endophytes inherit the medicinal properties from their host plant. Hence Published 1. 10. 2021 the detailed study of plant- microbe interaction is important. The anatomical features revealed the fungal mycelium in the ground cells of the abaxial midrib the adaxial segment of xylem in the midrib. In the of lamina fungal hyphae are seen in the epidermis and also in the mesophyll tissue beneath the epidermal layer. Three endophytic fungi namely Aspergillus niger Diaporthe longicolla and Regular article Schizophyllum commune were isolated and characterized using their ITS region. Keywords Endophytic fungi Alangium salviifolium light microscopy molecular characterization INTRODUCTION Sectioning Endophytes live inside the tissues of all plants without any sign of The paraffin embedded specimens were sectioned 10-12 µm thickness using diseases Petrini 1991 . Endophytes confer greater resistance to host against Rotary Microtome. Dewaxing of the
