In this study, some enzymology parameters and some antibiotics affecting the enzyme activity of 6- phosphogluconate dehydrogenase (6PGD) extracted from t the livers of fish from Lake Van (Chalcalburnus tarichi) were investigated because it is an important enzyme-producing NADPH, a reductive power, protecting the cell against the oxidative agents by producing reduced glutathione. The crude enzyme solution was obtained by using the affinity chromatography method. The native molecular weight of the enzyme is ∼90,000 ± 3000 Da and it is composed of two subunits with identical molecular weights of ∼46,000 Da as exhibited on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. |
