The thioredoxin system, found in all living creatures, consists of thioredoxin protein (Trx), thioredoxin reductase enzyme (TrxR), and NADPH. In this study, turkey liver mitochondrial TrxR enzyme with EU × mg −1 specific activity was purified 990-fold in a yield of using 2’,5’-ADP Sepharose 4B affinity chromatography. The purity of the enzyme was measured and the molecular weight of its subunits was determined to be kDa by SDSPAGE. The molecular mass of the enzyme’s natural state was found to be 88 kDa using Sephadex-G 150 gel filtration chromatography. |
