This strain also showed the lowest of doubling time. Ps. otitidis SE8 and OL2 had higher activity to solubilize phosphorus than other tested strains. Bacterial strain SE8 also gave a considerable amount of soluble potassium as compared with other strains being ppm. | Annals of Agricultural Science 2015 60 1 131 140 H O S T E D BY Faculty of Agriculture Ain Shams University Annals of Agricultural Science locate aoas Characterization of fluorescent pseudomonads isolates and their efficiency on the growth promotion of tomato plant Fekria . Saber a Ahmed A. Abdelhafez b Enas A. Hassan b Elshahat M. Ramadan b a Heliopolis University Biotechnology Dept. Egypt b Dept. of Agricultural Microbiology Faculty of Agriculture Ain Shams University Egypt Received 18 April 2015 accepted 28 April 2015 Available online 26 May 2015 KEYWORDS Abstract Soil samples were collected from different rhizosphere plants grown in SEKEM farm Fluorescent pseudomonads Bilbis El-Sharkyia governorate Egypt. Four fluorescent pseudomonads isolates out of seventy PGPR one were selected according to their efficiency to produce IAA cyanide antagonistic effect and Biocontrol high ability to solubilize potassium and phosphorus. These isolates were identified using 16S Soil-borne pathogens rRNA gene sequencing technique to be different strains of Pseudomonas otitidis. Growth curve 16S rRNA and growth kinetics were determined on king s broth medium. Ps. otitidis SE8 gave the highest Tomato specific growth rate multiplication rate and number of generation being h 1 h 1 and h 1 respectively. This strain also showed the lowest of doubling time. Ps. otitidis SE8 and OL2 had higher activity to solubilize phosphorus than other tested strains. Bacterial strain SE8 also gave a considerable amount of soluble potassium as compared with other strains being ppm. The maximum IAA and gibberellic acid production was also recorded by Pseudomonas SE8 and OL2. A remarkable quantity of siderophores was detected in the case of Ps. otitidis SE8 being mM DFOM. Application of these isolates as inoculants for tomato plants in green house was performed. The results showed that inoculation of tomato seedling with Pseudomonas strains led to suppress the .
