Da cá bò gai móc Monacanthus chinensis được xử lý để tách chiết collagen bằng phương pháp hóa học. Tạp chất phi collagen được xử lý bằng NaOH, sắc tố của da cá được loại bỏ bằng H2O2 và chiết tách collagen bằng acid acetic. Điều kiện xử lý và tách chiết collagen được tối ưu hóa bằng phương pháp bề mặt đáp ứng. Nồng độ, tỷ lệ da/dung dịch (g/ml) và thời gian ngâm được kiểm chứng thực nghiệm. | Vietnam Journal of Marine Science and Technology Vol. 20 No. 4A 2020 141 154 DOI https 1859-3097 15657 http jmst Optimizing conditions for treatment and extraction of collagen from fan- bellied leatherjacket skin Monacanthus chinensis Osbeck 1765 Doan Thi Thiet1 Pham Xuan Ky1 Phan Bao Vy1 Nguyen Phuong Anh1 Le Ho Khanh Hy1 Dao Viet Ha1 Vu Ngoc Boi2 1 Institute of Oceanography VAST Vietnam 2 Nha Trang University Khanh Hoa Vietnam E-mail doanthithiet671990@ Received 28 August 2020 Accepted 26 October 2020 2020 Vietnam Academy of Science and Technology VAST Abstract The fan-bellied leatherjacket skin was treated to extract collagen by chemical method. The non-collagenous substances and the pigment in the skin were removed with NaOH and H2O2 respectively. Collagen was extracted with acetic acid. The treatment and extraction conditions were optimized by response surface methodology. The concentrations skin solution ratios g ml and times were surveyed. The optimum conditions to remove the non-collagenous substances were as follows the concentration of NaOH at the ratio g ml at 1 time at hours. Using H 2O2 with the concentration at 6 the skin solution ratio g ml at 1 2 time at 10 minutes were suitable values for skin pigmentation removal. For collagen extraction the concentration of acetic acid at skin solution ratio at 1 g ml and time in hours were optimal. Keywords Chemical method fan-bellied leatherjacket skin non-collagenous substances pigment. Citation Doan Thi Thiet Pham Xuan Ky Phan Bao Vy Nguyen Phuong Anh Le Ho Khanh Hy Dao Viet Ha Vu Ngoc Boi 2020. Optimizing conditions for treatment and extraction of collagen from fan-bellied leatherjacket skin Monacanthus chinensis Osbeck 1765 . Vietnam Journal of Marine Science and Technology 20 4A 141 154. 141 Tạp chí Khoa học và Công nghệ Biển Tập 20 Số 4A 2020 141 154 DOI https 1859-3097 15657 http .
