The purpose of this study is to determine the content of adulterated protein enhancers, ammelide (AMD) and dicyandiamide (DCD), in animal feed according to the sample preparation process including the cleaning process by solid phase extraction (SPE) and quantification by HPLC-MS/MS. Method limit detection and quantitation values for both AMD and DCD were of 5 and 10 ppb, respectively. The good performance of the method in term of low threshold of detection, high sensitivity and reliability made it possible to be effectively applied at National Centre for Veterinary Drugs and Bio-Products Control to control the content of AMD and DCD in the basis of actual animal feed samples |
