In the present work "Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by a Moderate Halophile Yangia sp. ND199 Using Glycerol as a Carbon Source", we have evaluated the cell growth and PHA production by Yangia sp. ND199 on different carbon sources in the saline medium and subsequently continued the studies on PHA accumulation using glycerol as carbon source. | Appl Biochem Biotechnol DOI s12010-015-1479-4 Poly 3-Hydroxybutyrate-co-3-Hydroxyvalerate Production by a Moderate Halophile Yangia sp. ND199 Using Glycerol as a Carbon Source Doan Van-Thuoc amp Tran Huu-Phong amp Dang Minh-Khuong amp Rajni Hatti-Kaul Received 26 October 2014 Accepted 1 January 2015 Springer Science Business Media New York 2015 Abstract Yangia sp. ND199 a moderate halophile isolated from mangrove soil sample in Vietnam was found to accumulate poly 3-hydroxybutyrate-co-3-hydroxyvalerate PHBV from unrelated carbon sources in a medium with w v NaCl. Cultivation with glycerol as carbon source and yeast extract as nitrogen source resulted in maximum cell dry weight of g l and PHBV content of wt containing mol of 3HV after 40 h. The 3HV content of the PHBV was the highest during initial stages of copolymer production and decreased with increase in the copolymer amount with time but was not affected by changing the pH of the culture medium. Only homopolymer poly 3-hydroxybutyrate was synthesized when monosodium glutamate was used as the nitrogen source. Fed-batch cultivation of Yangia sp. ND199 with glycerol and yeast extract gave PHBV content and productivity of wt and g l h respectively which were reduced to wt and g l h respectively with crude glycerol as carbon source. Both the copolymer content and productivity were improved to 56 wt and g l h respectively by using 1 1 mixture of crude glycerol and high fructose corn syrup. This is the first report of PHBV production by a wild-type halophilic bacterium using glycerol as carbon source. Keywords Yangia sp. ND199 . Poly 3-hydroxybutyrate-co-3-hydroxyvalerate . Glycerol . Carbon source . 3HV content Introduction Polyhydroxyalkanoates PHAs are a group of biodegradable polyesters of biological origin which have attracted industrial interest as potential alternatives for fossil-based plastics 1 . PHAs D. Van-Thuoc T. Huu-Phong D. Minh-Khuong Department of .
