Định lượng đồng thời cortisol và testosterone trong huyết thanh chuột bằng sắc ký lỏng hiệu năng cao ghép nối đầu dò khối phổ nghiên cứu ảnh hưởng của saponin chi Panax đến nồng độ một số hormone trên động vật thí nghiệm. | VNU Journal of Science Medical and Pharmaceutical Sciences Vol. 38 No. 1 2022 45-53 Original Article Simultaneous Determination of Cortisol and Testosterone from Rat Serum by Liquid Chromatography-Tandem Mass Spectrometry Nguyen Van Khanh1 Nguyen Thị Thanh Binh1 Dang Kim Thu1 Dang Thao Linh1 Vu Thi Ngoc Anh1 Vu Thi Thu Giang2 Seijiro Honma3 1 VNU University of Medicine and Pharmacy 144 Xuan Thuy Cau Giay Hanoi Vietnam 2 Hanoi University of Pharmacy 13-15 Le Thanh Tong Hoan Kiem Hanoi Vietnam 3 Kanazawa University Kakumamachi Kanazawa Ishikawa 920-1192 Japan Received 19 February 2022 Revised 23 February 2022 Accepted 5 March 2022 Abstract A liquid chromatography-electrospray ionization tandem mass spectrometry in a positive mode LC-ESI-MS MS was developed to determine simultaneously the levels of cortisol and testosterone in rat serum. The MRM Multiple Reaction Monitoring was used to optimize ion precursor fragmentor voltage ion product and collision energy. Analytical samples were treated by solid-phase extraction with Bond Elut C18 cartridge. The method was validated according to FDA guidance for bioanalytical method validation. The results indicated that this method showed a wide linearity range ng mL for cortisol and ng mL for testosterone good linearity r2 gt low limit of quantification LLOQ values of cortisol and testosterone were ng mL and ng mL respectively good recovery - suitable repeatability CV values were in the range of - good stability in different conditions. The validated method was applied to quantify cortisol and testosterone levels in several rat blood samples. Keywords LC-ESI-MS MS cortisol testosterone rat serum solid-phase extraction. _ Corresponding author. E-mail address khanha7k64dkh@ https 2588-1132 45 46 N. V. Khanh et al. VNU Journal of Science Medical and Pharmaceutical Sciences Vol. 38 No. 1 2022 45-53 Định lượng đồng thời cortisol và .
