A high sensitive simultaneous determination method of nine steroid hormones including Estrone (E1), estradiol (E2), testosterone (T), dihydrotestosterone (DHT), 17-hydroxyprogesterone (17OH-P4), androstenedione (AN), progesterone (P4), dehydroepiandrosterone (DHEA), and cortisol by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESIMS/MS) was established in a positive mode using recently developed picolinyl derivatization. |