Bài viết Tạo cấu trúc biểu hiện và biến nạp gene GmDREB7A thông qua Agrobacterium ở thuốc lá đề cập đến kết quả thiết kế gen và vector biểu hiện mang gen GmDREB7A phục vụ nghiên cứu chức năng của GmDREB7A trong hệ gen đậu tương thông qua kỹ thuật chuyển gen. | TNU Journal of Science and Technology 227 10 17 - 25 GENERATING GENE EXPRESSION CONSTRUCT AND AGROBACTERIUM- MEDIATED TRANSFORMATION OF GmDREB7A IN TOBACCO Tu Quang Tan1 Bui Thi Minh Thuy1 Vanhsy Sysouphanh1 Nguyen Thi Hai Yen2 Nguyen Thi Ngoc Lan1 Chu Hoang Mau1 1TNU - University of Education 2TNU - University of Sciences ARTICLE INFO ABSTRACT Received 28 5 2022 Soybean is a seed crop with high economic and nutritional value and a soil improvement crop that poorly tolerates drought and salinity. In Revised 14 6 2022 the soybean genome the DREB gene group has been identified as Published 14 6 2022 activating transcription of stress-resistance genes including some genes of unknown function and DREB7 is one of them. In this study KEYWORDS the GmDREB7A gene and its expression construct were designed and successfully transformed into tobacco. Gene GmDREB7A includes an Drought resistance amino acid coding sequence a c-myc coding segment KDEL and an GmDREB7A gene additional short nucleotide at the 5 and 3 ends containing the restriction enzyme s cut-off point. Two transgenic constructs Gene expression construct pBI121_GmDREB7A and pZY_GmDREB7A have been successfully Salt resistance designed. The results of structural transformation pBI121_GmDREB7A Transgenic tobacco carrying the GmDREB7A gene into tobacco plants produced 248 transgenic plants after selection with kanamycin and 30 plants under greenhouse conditions with nine plants were positive for PCR. It is necessary to continue to analyze the T0 transgenic tobacco plants that are positive for PCR and evaluate the tolerance to drought and salinity stress of the GmDREB7A transgenic plants. TẠO CẤU TRÚC BIỂU HIỆN VÀ BIẾN NẠP GENE GmDREB7A THÔNG QUA AGROBACTERIUM Ở THUỐC LÁ Từ Quang Tân1 Bùi Thị Minh Thúy1 Vanhsy Sysouphanh1 Nguyễn Thị Hải Yến2 Nguyễn Thị Ngọc Lan1 Chu Hoàng Mậu1 1 Trường Đại học Sư phạm - ĐH Thái Nguyên 2Trường Đại học Khoa học - ĐH Thái Nguyên THÔNG TIN BÀI BÁO TÓM TẮT Ngày nhận bài 28 5 2022 Đậu tương
