Nghiên cứu nhân giống lan hài Chân tím (Paphiopedilum tranlienianum) bằng kỹ thuật in vitro

Bài viết Nghiên cứu nhân giống lan hài Chân tím (Paphiopedilum tranlienianum) bằng kỹ thuật in vitro tiến hành tái sinh phôi lan hài Chân tím trong môi trường nhân tạo nhằm mục đích tạo nguồn vật liệu cho nhân giống loài hoa đặc hữu có giá trị. | TNU Journal of Science and Technology 227 14 185 - 193 RESEARCH ON USING IN VITRO TECHNIQUES TO PROPAGATE LADY SLIPPER ORCHID PAPHIOPEDILUM TRANLIENIANUM Nguyen Thi Tinh1 2 Nguyen Tien Dung2 Bui Tri Thuc2 Duong Thi Thu Hoai2 Luu Hong Son2 Le Thanh Ninh2 Tran Ngoc Hung3 Ngo Xuan Binh2 1Vietnam Academy of Agricultural Sciences 2TNU - University of Agriculture and Forestry 3Fruit and Vegetable Research Institute ARTICLE INFO ABSTRACT Received 16 8 2022 The difference between lady s slipper orchids and others is easy to realize beause of the special morphology of flowers. There is only one Revised 15 9 2022 sac-like petal as a slipper in the flower therefore this orchids species Published 15 9 2022 named as quot lady s slipper orchids quot . Paphiopedilum tranlienianum is one of endemic orichids in Vietnam. The fruits which were pollinated KEYWORDS after 9 months were selected for experiments. The embryos were cultivated and germinated on MS medium supplemented with 30 g l Slipper Orchird - Paphiopedilum sugar 1 g l activated carbon g l agar 150 ml l coconut water. The malipoense rapid multiplication phase was supplemented with growth regulator In vitro culture as BAP 4 mg l or BAP 4 mg l combined with mg l kinetin. Bud regeneration In this media the hormone had a great influence on the ability to create many buds with the coefficient of germination is 79 Shoot NAA height is cm tree is fat and green. The root is induced the most PLBs in the MS medium which was supplemented with 30 g l sugar g l agar 150 ml l coconut water and 1 mg l concentration of NAA or IAA which can enhance the growth of root in vitro. NGHIÊN CỨU NHÂN GIỐNG LAN HÀI CHÂN TÍM PAPHIOPEDILUM TRANLIENIANUM BẰNG KỸ THUẬT IN VITRO Nguyễn Thị Tình1 2 Nguyễn Tiến Dũng2 Bùi Tri Thức2 Dương Thị Thu Hoài2 Lưu Hồng Sơn2 Lê Thanh Ninh2 Trần Ngọc Hùng3 Ngô Xuân Bình2 1Viện Khoa học Nông nghiệp Việt Nam 2Trường Đại học Nông lâm - ĐH Thái Nguyên 3Viện Nghiên cứu Rau Quả THÔNG TIN BÀI BÁO TÓM .