Striped catfish is one of the most imortant aquaculture species. Feed cost is a large component in striped catdfish production cost. Improving feed utilization is concerning to efficiency and sustainable aquaculture. Our general objective that improving feed utilization in striped catfish by addition of | 12/24/2008 Research institute for Aquaculture No2 116 Nguyen Dinh Chieu, Dist 1, HCM city STUDY ON PRODUCING BACTERIAL DIGESTIVE ENZYME FOR USE IN STRIPED CATFISH FEED TO IMPROVE FEED UTILIZATION Nguyen Van Nguyen , Bach Quynh Mai Phạm Duy Hai, Giap Văn Thang OUTLINE I. INTRODUCTION II. CONTENT III. MATERIAL & METHOD IV. RESULT & DISCUSSION V. CONCLUSION (5 sections) 1 12/24/2008 I. INTRODUCTION Currently, Striped catfish is one of the most important aquaculture species. Feed cost is a large component in striped catfish production cost. Improving feed utilization is concerning to efficiency and sustainable aquaculture • Consequently, our general objective that improving feed utilization in striped catfish by addition of bacterial digestive enzymes II. CONTENT 1. To establish the scheme to collect the crude enzyme extract from internal organ of striped catfish. 2. Determine the digestive enzyme activities in each organ of the tract of fish which range from (50gr, 300gr and 600gr). 3. Assessment of in vitro digestibility of inherent enzyme extract some ingredients and striped catfish feed 4. Assessment of in vitro digestibility of inherent enzyme extract combined with external enzyme on some ingredients and Tra feed and determine the ratio of external digestive enzyme to add in striped catfish feed . 5. Produce the bacterial digestive enzymes including protease, alpha amylase, phytase and beta glucanase to add in striped catfish feed. 6. Research on in vitro digestibility of the bacterial digestive enzyme on some ingredients and striped catfish feed 7. Evaluation of the bacterial digestive enzyme by striped catfish culture in tank and field study at intensive scale. (7items) • 2 12/24/2008 Approach Diagram Strippe Striped ingredi d catfish feed catfish ents Commercial Mixtu Protease re A Crude in vitro in vivo Field enzyme farming extract digest ib culture ivity commercial Mixtu Alpha amilase re B Mixture of preparation mixtu . E + .