Đông trùng hạ thảo là sản phẩm do nấm Cordyceps sinensis ký sinh và phát triển trong ấu trùng thuộc họ Hepialidae từ mùa đông đến mùa hè trên các vùng núi cao >3000m so với mặt biển ở Trung Quốc, Nepal Bài viết trình bày xác định sản phẩm đông trùng hạ thảo dựa vào chỉ thị ITS1 của nấm Codycepts sinensis và coi của ấu trùng bướm thuộc họ Hepialidae. | TNU Journal of Science and Technology 228 01 393 - 400 DETERMINATION OF PROPCESSED CATERPILLAR FUNGUS BASED ON ITS1 MARKERS ON CODYCEPTS SINENSIS AND COI ON GHOST MOTH LARVAE OF HEPIALIDAE FAMILY Le Quang Trung1 Tran My Linh2 Nguyen Chi Mai2 Nguyen Tuong Van3 1VNTEST Institute for Quality Testing and Inspection - Vietnam Union of Science and Technology Associations 2Institute of Marine Biochemistry VAST 3Institute of Biotechnology - VAST ARTICLE INFO ABSTRACT Received 31 10 2022 Products of caterpillar fungus are the complex of Cordyceps sinensis species parasitic to larvae of species of Hepialidae family and developing from winter Revised 07 12 2022 to summer in high mountains of China Nepal Caterpillar fungus has high Published 20 12 2022 economic benefits because of its medicinal properties. Therefore it could be mixed with clavicipitaceous fungi which are parasitic on larvae of different moth hosts and are produced throughout the year in natural condition or KEYWORDS artificial media. In this research 10 caterpillar fungus samples in capsules were Caterpillar fungus determination examined based on markers of ITS1 of Codycepts species and COI of Hepialidae and Bombyx mori larvae. Phylogenetic and homology level ITS markers of Codycepts fungi analyses based on nucleotide polymorphism of the markers revealed that 10 COI markers of Hepialidae larvae samples contained both caterpillar fungus of C. sinensis and clavicipitaceous Phylogenetic analysis fungi of Codycepts militaris and Metacodycepts neoginnii. Similarly powder of Hepialidae larvae was found in all 10 samples but five of which were also Homology level analysis containing that of B. mori. Therefore 10 samples were ditermined as no pure caterpillar fungus from C. sinensis and five of them were wrong about Hepialidae hosts. The results in this study suggest the effective uses of molecular markers in identifying possible sources of processed caterpillar fungus products. XÁC ĐỊNH SẢN PHẨM ĐÔNG TRÙNG HẠ THẢO .
