Bài viết Phân tích vùng gene lục lạp, rbcL, phân lập từ cây lan tai cáo (Hoya parasitica (Roxb.) Wall. ex Wight) được nghiên cứu nhằm mục đích khuếch đại và giải trình tự nucleotide của gene rbcL từ cây Lan tai cáo (H. parasitica), thiết lập cây phát sinh chủng loại, tìm kiếm chỉ thị cpDNA phục vụ định danh loài hoặc chi. | TNU Journal of Science and Technology 228 01 474 - 481 ANALYSIS OF THE CHLOROPLAST GENE REGION rbcL ISOLATED FROM Hoya parasitica Roxb. Wall. ex Wight PLANT Hoang Viet Cuong1 Mai Thi Hoang Anh1 Tu Quang Tan2 Chu Hoang Mau2 1Thai Nguyen Department of Education and Training 2TNU - University of Education ARTICLE INFO ABSTRACT Received 29 12 2022 Hoya parasitica Roxb. Wall. ex Wight an epiphytic species is an ornamental and precious medicinal plant. H. parasitica contains many Revised 30 01 2023 biologically active pharmaceutical substances that are used to treat some Published 31 01 2023 human diseases. H. parasitica is morphologically similar to some species of the Hoya genus and the Asclepiadaceae family. How to identify the species KEYWORDS and genera in the Asclepiadaceae family when the morphology growth and development of the plants are incomplete or the plant specimen is rbcL gene deformed or in powder form was the question posed for this study. In this Hoya parasitica work the rbcL gene was isolated and sequenced analyzed the genetic cpDNA barcoding relationship of H. parasitica was at the species and genera levels by PCR method nucleotide sequencing and molecular evolutionary analysis. The Phylogeny analysis showed that the rbcL gene has 547 nucleotides the coverage is Molecular Evolution from 99 - 100 and the similarity rate with the rbcL sequences of other species in the Asclepiadaceae family on GenBank ranges from - . The phylogenetic tree based on the rbcL sequence of species of the Hoya genus and species of the Asclepiadaceae family has been established. The rbcL gene sequence may be a candidate for chloroplast DNA barcoding for genera-level identification. PHÂN TÍCH VÙNG GENE LỤC LẠP rbcL PHÂN LẬP TỪ CÂY LAN TAI CÁO Hoya parasitica Roxb. Wall. ex Wight Hoàng Việt Cường1 Mai Thị Hoàng Anh1 Từ Quang Tân2 Chu Hoàng Mậu2 1Sở Giáo dục amp Đào tạo Thái Nguyên 2 Trường Đại học Sư phạm - ĐH Thái Nguyên THÔNG TIN BÀI BÁO TÓM TẮT Ngày nhận bài 29 12
