Bài viết Nghiên cứu tạo dòng và biểu hiện protein tái tổ hợp TS24 của Taenia spp nghiên cứu này chúng tôi đã tạo dòng và biểu hiện đoạn gene tái tổ hợp TS24 mã hóa protein TS24 của Taenia spp. để sử dụng như một nguồn kháng nguyên thay thế cho kháng nguyên thô. | Vietnam Journal of Community Medicine Vol. 64 Special Issue 2023 115-121 INSTITUTE OF COMMUNITY HEALTH CLONING AND EXPRESSION OF TS24 RECOMBINANT PROTEIN OF TAENIA SPP Nguyen Thi Van Anh Dang Trinh Minh Anh Pham Nguyen Thuy Vy Ngo Thi Hong Phuong Nguyen Thi Minh Chau Tran Thi Khanh Quynh Tran Minh Qui Nguyen Ho Quynh Ngan Nguyen Thi My Duyen Vo Huynh Minh Hien Institute of Malariology Parasitology and Entomology of HCMC - 685 Tran Hung Dao 1 ward 5 district Ho Chi Minh City Vietnam Received 08 12 2022 Revised 10 01 2023 Accepted 14 02 2023 ABSTRACT Background Cysticercosis is a parasitic tissue infection caused by larval cysts of the tapeworm Taenia solium. The patients often have few or no symptoms for many years. Currently serological tests are being increasingly and ELISA test is widely used for diagnosis. Nevertheless ELISA using native antigens had the problem with cross reactions with other parasites. Moreover the preparation of native antigens also had the limition of source of antigen. Therefore recombinant antigens have been decribed as an alternative to native antigens for diagnosis. Objective The purpose is to clone and express the TS24 recombinant protein of Taenia spp. to use it as an alterlative source in order to overcome the problems in using native antigens. Subject and method TS24 gene extracted from proglottid of Taenia spp. collected in Institute of Malariology - Parasitology - Entomology in HCM City encoding TS24 protein was amplified and cloned into the vector pET28a. Then the plasmid pET28a-TS24 was transformed into E. coli BL21 for expression. Results TS24 gene encoding TS24 recombinant protein had the size of 282 bp and had 92 5 identical to TS24 of T. solium on GenBank . TS24 gene was successfully subcloned to pET28a for expression. Finally TS24 recombinant protein was expressed at 37oC in 6h and had the size of 12 kDa. Conclusion The TS24 recombinant protein will be used as an antigen for developing of ELISA test for .
