Sodium metasilicate solution 60 g of sodium metasilicate and make up to 2 l with distilled water in a 2 l flask for lab up to 20 litres. Filter through micron filter paper and use 1ml/litre of water in lab; 180g of sodium metasilicate and make up to 1 litre with distilled water Then use 200ml/2 mt tank or 650ml/ tank | Algae Culture Manual Species for use 1. Chaetoceros gracilis Stock from USA and Thailand good for all stages of culture 2. Thalassiosira weissflogii Stock from USA especially for early zoea to help prevent Z2 syndrome Nutrients 1. AGP-C Algae Growth Promoter Complete Plates and tubes 250ppm ml litre 200 ml and 500 ml bottles 250ppm ml litre 2 litre and 20 litre bottles 100ppm ml 2 litres 2 ml 20 litres 2 mt tanks 25ppm 50ml 2 mt tank mt tanks 15ppm ml mt tank 2. Sodium metasilicate solution 60 g of sodium metasilicate and make up to 2 l with distilled water in a 2 l flask for lab up to 20 litres. Filter through micron filter paper and use Iml litre of water in lab 180g of sodium metasilicate and make up to 1 litre with distilled water Then use 200ml 2 mt tank or 650ml tank. 3. EDTA 10ppm of EDTA either powder or preferably Verses 100 for all cultures 65 g ml mt tank 20g ml 2 mt tank ml 20 litre bottle. 4. Vitamin B mixture B1 B6 B12 Put 1 vial into 100 ml of autoclaved distilled water Use at litre 200 ml 500ml 2 litre or 2ml 20 litre bottle. Do not use for 2mt or tanks 5. Tris buffer solution Tris 50 g distilled water 250ml in a 500ml flask - shake to dissolve 6. HCl solution 37 concentrated Hydrochloric acid HCL and a plastic Pasteur pipette to use as a dropper 7. Liquid Chlorine Sodium hypochlorite solution 8-10 active chlorine solution. Used in all tanks from 2 litres upwards at 10ppm active chlorine 100ml mt litre to give 10ppm and left without air overnight then aerated and treated with sodium thiosulphate to dechlorinate. 1 8. Sodium Thiosulphate solution 250 g Sodium thiosulphate crystals made up to 1 litre of distilled water in a 1 litre flask and shaken to dissolve. 9. Neutralization of chlorine Volume of sodium thiosulphate solution ml ppm chlorine volume litres 250 Cleaning and sterilizing equipment Cleaning All equipment Petri dish tubes flasks pipettes funnels etc must
