Introduction: Propionic acid derivative analgesic-antipyretics ( Table ) are non-steroidal and antiinflammatory drugs. As one of mechanisms of their pharmacological actions, inhibition of prostaglandin biosynthesis can be mentioned. Although fatal cases due to propionic acid derivative analgesic-antipyretics are not many in the world, the incidence of poisoning (including survived cases) by this drug group is relatively high among therapeutic drugs in Japan [1]. Analyses of propionic acid derivative analgesic-antipyretics are being made by TLC [2, 3], HPLC [2, 4–8], GC [9], GC/MS [10] and LC/MS [11]. In this chapter, the methods of analysis of this drug group by TLC,. | Propionic acid derivative analgesic-antipyretics by Tatsuo Shinozuka and Rika Nakajima Introduction Propionic acid derivative analgesic-antipyretics Table are non-steroidal and antiinflammatory drugs. As one of mechanisms of their pharmacological actions inhibition of prostaglandin biosynthesis can be mentioned. Although fatal cases due to propionic acid derivative analgesic-antipyretics are not many in the world the incidence of poisoning including survived cases by this drug group is relatively high among therapeutic drugs in Japan 1 . Analyses of propionic acid derivative analgesic-antipyretics are being made by TLC 2 3 HPLC 2 4-8 GC 9 GC MS 10 and LC MS 11 . In this chapter the methods of analysis of this drug group by TLC HPLC and GC are presented. TLC analysis Reagents and their preparation Ibuprofen ketoprofen naproxen fenoprofen calcium flurbiprofen loxoprofen and oxaprozin can be purchased from Sigma St. Louis MO USA . For other drugs pure powder of each drug can be obtained by direct request to each manufacturer as follows alminoprofen from Maruho Pharmaceutical Co. Ltd. Tokyo Japan zaltoprofen from Japan Chemi-phar Pharmaceutical Co. Ltd. Tokyo Japan thiaprofenic acid from Aventis Pharma Strasbourg France pranoprofen from Mitsubishi Welpharma Osaka Japan. High-performance HP TLC platea silica gel 60 F254 HPTLC Merck Darmstadt Germany . Dichloroindophenol reagent g of 2 6-dichloroindophenol sodium Sigma is dissolved in 50 mL ethanol. Bromocresol green reagent g of bromocresol green sultone form Sigma is dissolved in 100 mL of 96 ethanol. Dragendorff reagent g of bismuth subnitrate is dissolved in a mixture of 40 mL distilled water and 10 mL acetic acid to prepare A solution. A 8-g aliquot of potassium iodide is dissolved in 20 mL distilled water to prepare B solution. Then a mixture of A B acetic acid distilled water 1 1 4 20 v v is prepared. Ninhydrin reagent g of ninhydrin is dissolved in a mixture of 5 mL acetic acid .
