Molecular Biology Problem Solver 7. Sách được nhiều nhà khoa học có uy tín, nhiều kinh nghiệm trong nghiên cứu thực nghiệm trình bày những vấn đề thường hay phát sinh trong phòng thí nghiệm. Do vậy mà sách không trình bày các protocol hay quy trình như các sách khác, thay vào đó các tác giả sẽ trình bày các vấn đề nhằm giúp giúp đọc giả: Tự nâng cao khả năng chẩn đoán nguyên nhân khi gặp các vấn để về kỹ thuật, quy trình, hóa chất, thuốc thử trong quá trình thực nghiệm trong phòng thí. | Does Absorbance Always Correlate with Concentration . 104 Why Does Popular Convention Recommend Working Between an Absorbance Range of to at 260 nm When Quantitating Nucleic Acids and When Quantitating Proteins at 280nm . 105 Is the Ratio A260 A280 a Reliable Method to Evaluate Protein Contamination within Nucleic Acid Preparations . 106 What Can You Do to Minimize Service Calls . 106 How Can You Achieve the Maximum Lifetime from Your Lamps . 107 The Deuterium Lamp on Your UV-Visible Instrument Burned Out. Can You Perform Measurements in the Visible Range . 107 What Are the Strategies to Determine the Extinction Coefficient of a Compound . 108 What Is the Extinction Coefficient of an Oligonucleotide . 108 Is There a Single Conversion Factor to Convert Protein Absorbance Data into Concentration . 108 What Are the Strengths and Limitations of the Various Protein Quantitation Assays . 109 Bibliography . 110 BALANCES AND SCALES Trevor Troutman How Are Balances and Scales Characterized Balances are classified into several categories. Top-loaders are balances with g or 1 mg readability and above where readability is the lowest possible digit that is seen on the display. Analytical balances are instruments that read . Semimicro balances are those that are mg. Microbalances are 1 mg. Finally the ultramicrobalances are mg. How Can the Characteristics of a Sample and the Immediate Environment Affect Weighing Reproducibility Moisture Condensation forms on reagents that are not kept airtight while they equilibrate to room temperature. Similarly moisture is generated if the sample is not allowed to reach the temperature of the weighing instrument. This is especially problematic when weighing very small samples. You the researcher are also a source of moisture that can be transmitted quite easily to the sample in the form of fingerprints and body oils. How to Properly Use and Maintain Laboratory Equipment 51 Air Buoyancy Akin to water keeping .
