Molecular Biology Problem Solver 8. Sách được nhiều nhà khoa học có uy tín, nhiều kinh nghiệm trong nghiên cứu thực nghiệm trình bày những vấn đề thường hay phát sinh trong phòng thí nghiệm. Do vậy mà sách không trình bày các protocol hay quy trình như các sách khác, thay vào đó các tác giả sẽ trình bày các vấn đề nhằm giúp giúp đọc giả: Tự nâng cao khả năng chẩn đoán nguyên nhân khi gặp các vấn để về kỹ thuật, quy trình, hóa chất, thuốc thử trong quá trình thực nghiệm trong phòng thí. | convert protocols between rotor types fixed-angle horizontal and vertical . Should Flamables Explosive or Biohazardous Materials Be Centrifuged in Standard Centrifugation Equipment Centrifuge manufacturers strongly recommend that standard laboratory centrifuges should not be exposed to any materials capable of producing flammable or explosive vapors or extreme exothermic reactions. Specialized equipment exists for centrifuging dangerous substances. Which Centrifuge Tube Is Appropriate for Your Application A broken or leaking sample container can seriously damage a centrifuge by knocking the rotor out of balance or exposing mechanical and electrical components to harsh chemicals. Damage can occur at any speed. Use only the tubes that are recommended for centrifugation use. If unsure contact the tube manufacturer to assess compatability. With the trend toward smaller sample size and greater throughput microplates have become very popular. Other protocols call for vials and slides. Never attempt to create your own adapter for these containers ask the rotor manufacturer about the availability of specialized equipment. Fit Correct tube fit is critical especially at higher g forces. Tubes or containers that are too large can get trapped in rotors while tubes that fit loosely can leak or break. Never use homemade adapters. While a broken tube doesn t sound costly poorly fitted containers can lead to costly repairs. g Force Many tubes are not suitable for high stress centrifugation. When in doubt about g force limitations contact the tube s manufacturer. If this isn t feasible you can test the tube by filling it with water centrifuging at low rpm s and inspecting the tube for damage or indications of stress while slowly increasing the speed. Chemical Compatibility Confirm the tube s resistance with the manufacturer. Containers that are not resistant to the sample might survive one or more centrifugations but will surely be weakened. Chemically resistant containers should .
