Molecular Biology Problem Solver 15. Sách được nhiều nhà khoa học có uy tín, nhiều kinh nghiệm trong nghiên cứu thực nghiệm trình bày những vấn đề thường hay phát sinh trong phòng thí nghiệm. Do vậy mà sách không trình bày các protocol hay quy trình như các sách khác, thay vào đó các tác giả sẽ trình bày các vấn đề nhằm giúp giúp đọc giả: Tự nâng cao khả năng chẩn đoán nguyên nhân khi gặp các vấn để về kỹ thuật, quy trình, hóa chất, thuốc thử trong quá trình thực nghiệm trong phòng thí. | Quaternary ammonium compounds. These compounds have detergent activity and solubilize cell membranes. They are commonly used at concentrations of 1 or less to disinfect floors benchtops and other inanimate objects. They are inexpensive odorless and nontoxic but they are not as microbicidal as the aforementioned compounds. Is It Necessary to Decontaminate Yourself or Your Clothing Is There Significant Risk of Contaminating Others The answer to both of the above questions is a resounding yes This is particularly important in a hospital setting where you will likely encounter patients that may be highly susceptible to infection. Recent studies Neely and Maley 2000 have shown that gram-positive bacteria particularly multidrug-resistant Staphylococcus aureus and vancomycin-resistant enterococcus can survive for prolonged time periods on common hospital and laboratory fabrics. Of 22 different organisms tested all survived at least a day and some for more than 90 days This study alone provides a good reason not to wear your lab coat to the cafeteria. Leave your lab wear including lab coats in the lab. Get your coat laundered with regularity. Any article of clothing whether lab attire or personal clothing should be washed very soon after an accidental spill of a microbial suspension or contaminated material onto it. Even if you have been wearing latex gloves wash your hands when finished working. These simple control procedures will go a long way to stop the spread of infection to yourself and to those around you. MEDIA PREPARATION AND STERILIZATION How Can You Work Most Efficiently with Your Media Preparation Group Learn the Capabilities and the Guidelines Media preparation facilities serve a crucial role for large numbers of researchers and they are usually extremely busy. The more familiar you become with the operational guidelines and functional capabilities of the facility the more likely you will get the materials you need when you need them. Get to Know the Staff .