Molecular Biology Problem Solver 16. Sách được nhiều nhà khoa học có uy tín, nhiều kinh nghiệm trong nghiên cứu thực nghiệm trình bày những vấn đề thường hay phát sinh trong phòng thí nghiệm. Do vậy mà sách không trình bày các protocol hay quy trình như các sách khác, thay vào đó các tác giả sẽ trình bày các vấn đề nhằm giúp giúp đọc giả: Tự nâng cao khả năng chẩn đoán nguyên nhân khi gặp các vấn để về kỹ thuật, quy trình, hóa chất, thuốc thử trong quá trình thực nghiệm trong phòng thí. | Your Count-Rate Meter Detects Radiation on the Outside of a Box Containing I mCi of a 32P Labeled dATP Is It Contaminated . I52 You Received 250 mCi of 32P and the Box Wasn t Labeled Radioactive. Isn t This a Dangerous Mistake . I53 Designing Your Experiments . I53 How Do You Determine the Molarity and Mass in the Vial of Material . I53 How Do You Quantitate the Amount of Radioactivity for Your Reaction . I55 Storing Radioactive Materials. I56 What Causes the Degradation of a Radiochemical . I56 What Can You Do to Maximize the Lifetime and Potency of a Radiochemical . I56 What Is the Stability of a Radiolabeled Protein or Nucleic Acid . I57 Radioactive Waste What Are Your Options and Obligations . I58 Handling Radioactivity Achieving Minimum Dose . I59 How Is Radioactive Exposure Quantified and What Are the Allowable Doses . I59 Monitoring Technology What s the Difference between a Count-Rate Counter and a Dose-Rate Meter . I59 What Are the Elements of a Good Overall Monitoring Strategy . I6I What Can You Do to Achieve Minimum Radioactive Dose . I62 How Can You Organize Your Work Area to Minimize Your Exposure to Radioactivity . I64 How Can You Concentrate a Radioactive Solution . I64 Bibliography . I66 Appendix A Physical Properties of Common Radionuclides . I66 The information within this chapter is designed as a supplement not a replacement to the training provided by your institutional rules and or radiation safety officer. At the very least there are some 10 fundamental rules to consider when working with radioactivity Amerhsam International 1974 1. Understand the nature of the hazard and get practical training. 2. Plan ahead to minimize time spent handling radioactivity. I42 Volny Jr. 3. Distance yourself appropriately from sources of radiation. 4. Use appropriate shielding for the radiation. 5. Contain radioactive materials in defined work areas. 6. Wear appropriate protective clothing and dosimeters. 7. Monitor the work area frequently for contamination .
