Molecular Biology Problem Solver 18. Sách được nhiều nhà khoa học có uy tín, nhiều kinh nghiệm trong nghiên cứu thực nghiệm trình bày những vấn đề thường hay phát sinh trong phòng thí nghiệm. Do vậy mà sách không trình bày các protocol hay quy trình như các sách khác, thay vào đó các tác giả sẽ trình bày các vấn đề nhằm giúp giúp đọc giả: Tự nâng cao khả năng chẩn đoán nguyên nhân khi gặp các vấn để về kỹ thuật, quy trình, hóa chất, thuốc thử trong quá trình thực nghiệm trong phòng thí. | What Can You Do to Achieve Minimum Radioactive Dose Attitude Consider the benefits of an attitude whereby everyone working with radioactivity continuously ponders if they are working in the safest most efficient manner. Is a particular radioactive experiment necessary Can the amount of radioactivity in an experiment be reduced Is there a faster safer way to carry out the work Questions like these will reduce cost and radioactive exposure. An institution s RSO is also required to implement a continuing education program regarding the principles of keeping personnel exposure dose low. If you find yourself becoming stressed while handling radioactivity or if that incessant clicking sound of the count-rate meter is causing a heightened sense of alarm you can always step away from the bench to put things into perspective. Estimate how much dose you are receiving from your activities and relate those back to your annual allowable dose. Time Work quickly and neatly. In the example above a finger lingering for 1 minute over an open 1 mCi vial of 32P will receive 17mrem whereas a 10 second exposure receives a sixfold lower dose. Practicing the manipulations of your experiments with nonradioactive materials will identify problem areas and ultimately enable you to work faster and safer. Working with radioactivity while feeling panicked or rushed will slow you down or cause an accident. If you can t smoothly do the motion in 10 seconds take 20. You ll improve through time and all along will be well aware of your estimated dose. You ll automatically be striving to lower your dose. Distance Dosage decreases with distance. Why A radiation source is like a light bulb. As the rays radiate outward in a sphere they cover a wider area but become less potent at any single point. Use the inverse square law to your advantage. Can you pipette with a longer pipettor Can you place the reaction vial even a few inches farther from you and others in the lab Can you place a film cassette .
