Molecular Biology Problem Solver 56. Sách được nhiều nhà khoa học có uy tín, nhiều kinh nghiệm trong nghiên cứu thực nghiệm trình bày những vấn đề thường hay phát sinh trong phòng thí nghiệm. Do vậy mà sách không trình bày các protocol hay quy trình như các sách khác, thay vào đó các tác giả sẽ trình bày các vấn đề nhằm giúp giúp đọc giả: Tự nâng cao khả năng chẩn đoán nguyên nhân khi gặp các vấn để về kỹ thuật, quy trình, hóa chất, thuốc thử trong quá trình thực nghiệm trong phòng thí. | in protein sample electrophoresis 354 for pulsed field electrophoresis 246 247 in quantitating nucleotide solutions 274 selecting 33-35 for sequential double digests 243-244 in simple digests 238-239 for simultaneous double digests 242 staining and 362 from stock solutions 36-37 storage lifetimes of 37-38 substitutions among 32 troubleshooting PCR 320 types of hybridization 427-430 unreliable 35-37 uses of 32 for Western blotting washing 382 Buffer salts 33 buffer reliability and 35 for pH standards 84 Burns 120 123-124 Calibration. See also Accuracy of balances and scales 55 of pH meters 81 82-83 83-84 87-89 90 92 of pipettes 68 70-77 of spectrophotometers 96-97 98-100 of storage phosphor imagers 444 Callbacks from suppliers 27-28 Calomel reference systems for pH meters 77-78 Candida albicans as biohazard 115 128 Carbohydrates autoclaving of 139 Carbon radioisotopes autoradiography film and 438 439 as radioactive waste 158 Casein as blocking agent 381 Catalyst concentration in acrylamide polymerization 343 344 Catalyst potency in acrylamide polymerization 342-343 Cathodic drift with gradient gels 347 cDNA in eukaryotic expression 497-498 cDNA clones in RNA purification 200 cDNA libraries 199 210 497-498 in RNA purification 201 CellCube in eukaryotic expression 514 Cell cultures for eukaryotic expression 514 for pulsed field electrophoresis 245-246 Cells. See also Host cells isolating DNA from 172-184 total RNA isolation from 203-206 total RNA yield from 201-202 Cellulose in RNA purification 210-212 Centers for Disease Control and Prevention CDC 115 Centrifugation types of 55-56 Centrifugation time calculating 59-61 Centrifuges 55-67 brakes for 62-63 hazardous materials in 61 maintenance of 62 refrigerated 66 rotors for 57 58-61 62-63 64 selecting 57 service calls for 64-66 spills in 66 troubleshooting 64-67 tubes for 61 walking 66 Certificate of Analysis for restriction endonucleases 228 232-233 with simple digests 238 Cesium chloride CsCl in plasmid purification
