Handbook of Microbiological Media, Fourth Edition part 2 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Composition of Media 5 Legionella BCYE Growth Supplement ACES buffer KOH ferric pyrophosphate L-cysteine-HCl and a-ketoglutarate. For the enrichment ofLegionella species. Leptospira Enrichment Lyophilized pooled rabbit serum containing hemoglobin that provides long-chain fatty acids and B vitamins for growth of Leptospira species. Metals 44 ZnSO4-7H2O FeSO4-7H2O MnSO4-7H2O Na2B4O7-10H2O CuSO4-5H2O Co NO3 26H2O and EDTA. Middlebrook ADC Enrichment NaCl bovine albumin fraction V glucose and catalase. The albumin binds free fatty acids that may be toxic to mycobacteria. Middlebrook OADC Enrichment NaCl bovine albumin glucose oleic acid and catalase. The albumin binds free fatty acids that may be toxic to mycobacteria the enrichment provides oleic acid used by Mycobacterium tuberculosis for growth. Mycoplasma Enrichment without Penicillin Horse serum fresh autolysate of yeast-yeast extract and thallium acetate. Provides cholesterol and nucleic acids for growth of Mycoplasma species. The thallium selectively inhibits other microorganisms. Mycoplasma Supplement Yeast extract and horse serum. Nitsch s Trace Elements MnSO4 H3BO3 ZnSO4 Na2MoO4 CuSO4 CoCl26H2O and H2SO4. Oleic Albumin Complex NaCl bovine albumin fraction V and oleic acid. The albumin binds free fatty acids that may be toxic to mycobacteria and the enrichment provides oleic acid that is used by Mycobacterium tuberculosis for growth. PPLO Serum Fraction Serum fraction A. Rabbit Blood Citrated Rabbit blood washed and treated with sodium citrate as an anticoagulant. Rabbit Blood Defibrinated Rabbit blood treated to denature fibrinogen without causing cell lysis. RPF Supplement Fibrinogen rabbit plasma trypsin inhibitor and potassium tellurite. For the selection and nutrient supplementation of Staphylococcus aureus. Sheep Blood Citrated Sheep blood washed and treated with sodium citrate as an anticoagulant. Sheep Blood Defibrinated Sheep blood treated to denature fibrinogen without causing cell lysis. SLA Trace .
