Handbook of Microbiological Media, Fourth Edition part 3 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | AAHC Medium for YAK Clones 15 Nicotinamide adenine Fe NO3 p-Aminobenzoic Vitamin Preparation of CVA Enrichment Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. i -Cysteine-HCTH2O Solution Composition per Preparation of i -Cysteine-HCTH2O Solution Add l-cysteine-HCl-H2O solution to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Urea Solution Composition per Urea Preparation of Urea Solution Add urea to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Aseptically combine of cooled sterile agar base and of sterile supplement solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and differentiation of Ureaplasma urealyti-cum from urine based on its ability to produce ammonia from urea. Bacteria that produce ammonia appear as golden to dark brown colonies. Also used for the cultivation of other Ureaplasma species. A 8B Agar Composition per Agar Supplement pH at 25 C Agar Base Composition per Pancreatic digest of Papaic digest of soybean Preparation of Agar Base Add components except agar to distilled deionized water and bring volume to . Adjust pH to . Add agar. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Supplement Solution Composition per Horse serum Fresh yeast extract Penicillin Urea L-Cysteine-HCl-H2O GHL tripeptide Preparation of Supplement Solution Aseptically combine
