Handbook of Microbiological Media, Fourth Edition part 9 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Alkaline Peptone Salt Broth 75 Alkaline Cellulose Agar Composition per liter Solution Solution Solution A Composition per Cellulose powder MN Yeast Preparation of Solution A Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to with 1jV HCl. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Solution B Composition per Preparation of Solution B Add of Na2CO3 to distilled deion-ized water and bring volume to . Mix thoroughly. Adjust pH to with 6 NaHCO3 solution. Autoclave for 15 min at 15 psi pres-sure-121 C. Cool to 50 -55 C. Preparation of Medium Aseptically combine of solution A with of solution B. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of cellulose-utilizing bacteria. Alkaline HiVeg Peptone Water Composition per liter Plant pH at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Distribute into tubes or flasks. Autoclave for 10 min at 15 psi pressure-121 C. Use For the enrichment of Vibrio species from foods. Alkaline Nutrient Agar Composition per liter Beef pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically adjust to pH with sterile 9 Na2CO3 solution. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of Bacillus alcalophilus and Bacillus species. Alkaline .
