Handbook of Microbiological Media, Fourth Edition part 21 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | BAM SMAgar Modified 195 Filter. To filtrate add an equal volume of potassium phosphate buffer pH . Add of dried liver concentrate. Mix thoroughly. Distribute into tubes or flasks in volumes. Autoclave for 20 min at 15 psi pressure-121 C. Prior to inoculation add of rice starch to each tube. Use For the cultivation and maintenance of Entamoeba histolytica and other intestinal protozoa. BAM Agar ATCC Medium 1655 Composition per liter Yeast NH4 Trace elements . pH at 25 C Trace Elements Composition per liter Preparation of Trace Elements Add components to of dis-tilled deionized water. Mix thoroughly. Preparation of Medium Add components except agar to dis-tilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Adjust medium to pH with H2SO4. Add agar to of distilled deionized water. Autoclave agar separately to avoid acid hydrolysis. Autoclave for 15 min at 15 psi pres-sure-121 C. Mix the two solutions together. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and maintenance of Bacillus acidoterrestris. BAM Broth Composition per liter Yeast NH4 Trace elements . pH at 25 C Trace Elements Composition per liter H3BO3 . Preparation of Trace Elements Add components to of dis-tilled deionized water. Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Adjust medium to pH with H2SO4. Distribute into tubes or flasks. Autoclave
