Handbook of Microbiological Media, Fourth Edition part 29 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | BS Medium 275 Preparation of Mineral Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Na2CO3 Solution Composition per Preparation of Na2CO3 Solution Add Na2CO3 to O2-free dis-tilled deionized water. Mix thoroughly. Gas with 100 CO2 for 15 min. Autoclave for 15 min at 15 psi pressure-121 C. Hemin Solution Composition per NaOH solution . Preparation of Hemin Solution Add hemin to ofNaOH solution. Mix thoroughly. L-Cysteine-HCl-Na2S Solution Composition per Preparation of L-Cysteine-HCl-Na2S Solution Add l-cysteine-HCl to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to 11 with NaOH. Add Na2S-9H2O. Mix thoroughly. Bring volume to with distilled deionized water. Gently heat and bring to boiling under 100 N2. Cool to 25 C under 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Vitamin Solution Composition per Calcium p-Aminobenzoic Folic Vitamin Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. VFA Volatile Fatty Acid Solution Composition per liter Acetic DL-a-Methylbutyric Isovaleric -Valeric Isobutyric Preparation of VFA Solution Add components to distilled deion-ized water and bring volume to . Mix thoroughly. Preparation of Medium Add components except L-cysteine-HCl-Na2S solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Continue boiling until resa-zurin turns colorless indicating reduction. Anaerobically distribute into tubes in volumes. Cap with butyl rubber .
