Handbook of Microbiological Media, Fourth Edition part 35 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Cell Growth Medium 335 Preparation of Crystal Violet Solution Add Crystal Violet to of distilled deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Cefsulodin Solution Composition per Preparation of Cefsulodin Solution Add cefsulodin to of distilled deionized water. Mix thoroughly. Filter sterilize. Novobiocin Solution Composition per Preparation of Novobiocin Solution Add novobiocin to of distilled deionized water. Mix thoroughly. Filter sterilize. Strontium Chloride Solution Composition per Preparation of Strontium Chloride Add strontium chloride to of distilled deionized water. Mix thoroughly. Filter sterilize. Preparation of Medium Add irgasan solution to basal medium. Mix thoroughly. Cool to 50-55 C. Add des-oxychlolate solution. Mix thoroughly. Solution should remain clear. Aseptically add 5N NaOH Neutral Red solution Crystal Violet solution cefsulodin solution and novobiocin solution. Mix thoroughly. Slowly add strontium chloride solution while continuously stirring. Adjust pH to with 5N NaOH. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the selective isolation and differentiation of Yersinia entero-colitica based on mannitol fermentation. Yersinia enterocolitica appears as bull s eye colonies with deep red centers surrounded by a transparent periphery. Cefsulodin Irgasan Novobiocin Agar See CIN Agar Celery Decoction Agar Composition per liter Potatoes infusion Celery decoction. pH at 25 C Celery Decoction Composition per Celery leaves and Preparation of Celery Decoction Coarsely cut celery into pieces. Add celery pieces to of distilled deionized water. Puree in a blender at high speed for 2 min. Strain through a wire sieve. Gently heat and .
