Handbook of Microbiological Media, Fourth Edition part 37 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Chloroflexus Agar 355 Trace Elements Solution SL-8 Composition per liter Disodium Preparation of Trace Elements Solution SL-8 Add components to distilled deionized water and bring volume to . Mix thoroughly. Na2S-9H2O Solution Composition per Preparation of Na2S-9H2O Solution Add Na2S-9H2O to distilled deionized water and bring volume to . Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. NaHCO3 Solution Composition per Preparation of NaHCO3 Solution Add NaHCO3 to distilled de-ionized water and bring volume to . Mix thoroughly. Filter sterilize. Vitamin B12 Solution Composition per Vitamin Preparation of Vitamin B12 Solution Add vitamin B12 to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium To of cooled sterile solution 1 aseptically add of sterile Na2S-9H2O solution of sterile NaHCO3 solution and of sterile vitamin B12 solution. Mix thoroughly. Adjust pH to with sterile H2SO4 or Na2CO3. Aseptically distribute into sterile or bottles with metal screwcaps and rubber seals. Completely fill bottles with medium except for a pea-sized air bubble. Use For the isolation and cultivation of freshwater and soil members of the Chlorobiaceae. Chlorobium thiosulfatophilum Medium Composition per Solution Solution Solution Trace elements pH at 25 C Solution A Composition per Preparation of Solution A Add NaHCO3 to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Solution B Composition per Preparation of Solution B Add Na2S-9H2O to distilled .
